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At each MBSS sampling location, benthic macroinvertebrates are sampled from a 20 ft2 area with a standard D-net (Stranko et al., 2014). Community composition data is used to calculate a suite of summary metrics that are included in the calculation of a B-IBI for the Coastal Plain region (Southerland et al., 2005). Established criteria are then used to score the metrics 1, 3, or 5, and the average of all individual metric scores represents the B-IBI (Stribling et al., 1998). The MBSS B-IBI scores can be further classified into four condition ranges: \u201cGood \u201c(≥4.0), \u201cFair\u201d (3.0-3.9), \u201cPoor\u201d (2.0-2.9), and \u201cVery Poor \u201c(1.0-1.9). 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