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The goals of the MBSS, which began in 1995, are to assess current condition and changes in ecological resources of Maryland\u2019s 1st through 4th order non-tidal streams, as well as provide an inventory of biodiversity and identify effects of stressors on the natural resources (Stranko et al., 2014). The MBSS is a stratified random survey with a lattice design. Year and basin are included as strata and sampling has been restricted to approximately one-third of the major drainage basins each year (Mercurio et al., 1999). An electrofishing component is utilized to survey fish communities. Community composition data is used to calculate a suite of summary metrics that are included in the calculation of a F-IBI for the Coastal Plain region (Southerland et al., 2005). Established criteria are then used to score the metrics 1, 3, or 5, and the average of all individual metric scores represents the F-IBI (Stribling et al., 1998). 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This is considered historic oyster bottom as charted prior to the present, legally designated Natural Oyster Bars, using source materials from 1906 to 1977 including the bars from Yates Survey (1906-1912). The layer and its development are described in a 1997 report (Smith et al. 1997), available at http://dnr2.maryland.gov/fisheries/Pages/oysters/historic-oyster-bottom.aspx , and a state-wide version of the layer is available from Maryland\u2019s Mapping and GIS Data Portal (MD iMAP) as \u201cMaryland Shellfish - Historic Oyster Bottom\u201d. There are two caveats to keep in mind: 1) The Yates Survey was conducted to define regulatory boundaries at that time, not necessarily to map all oyster habitat, so comparisons between this layer and modern habitat surveys are not warranted; and 2) The layer is historic \u2013 it does not depict the boundaries of the current legally recognized Natural Oyster Bars. 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